364 The neuropeptide B type natriuretic peptide (BNP) induces limited activation of human monocyte-derived Langerhans cells

B type natriuretic peptide (BNP) has emerged as a central signaling molecule in itch transduction, first identified this context neurotransmitter the nervous system, but lately also epidermal compartment paracrine mediator. Pruritus is most common symptom dermatology and especially chronic inflammatory skin diseases such atopic dermatitis, prurigo nodularis psoriasis, where BNP levels have been reported to be increased. Its action on nerves increasingly well documented, little known about its effect immune cells skin. Specifically, Langerhans (LCs), sole professional antigen presenting cell population steady state epidermis, not investigated. As LCs are commonly found associated with nerve endings our current experiments we aimed at uncovering of human monocyte-derived using RNASeq. Our previous results showed that express receptor NPR1, activation stimulates expression CD207 (Langerin) these cells. RNASeq analysis treated relatively few biological processes were upregulated (Positive regulation T activation, Cytokine-cytokine interaction, Dendritic spine, Calcium channel complex, Regulation tolerance), only one was downregulated (Cell-cell junction organization). We contrasted activated by TLR7/8 synthetic agonist CL075, which more profile (upregulated pathways: IL-4 IL-13 signaling, IL-10 signaling; Macrophage migration, spine). Based might counterbalance classical stimuli.